General Info
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This Retn Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
Information
| Gene Symbol: | Retn |
| Gene ID: | 57264 |
| Uniprot ID: | RETN_MOUSE |
| Specificity: | This kit recognizes Mouse RETN in samples. No significant cross-reactivity or interference between Mouse RETN and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Function | Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. |
| Protein Name | ResistinAdipose Tissue-Specific Secretory FactorAdsfAdipose-Specific Cysteine-Rich Secreted Protein A12-AlphaCysteine-Rich Secreted Protein Fizz3 |
| Database Links | Reactome: R-MMU-6798695 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Resistin ELISA kitAdipose Tissue-Specific Secretory Factor ELISA kitAdsf ELISA kitAdipose-Specific Cysteine-Rich Secreted Protein A12-Alpha ELISA kitCysteine-Rich Secreted Protein Fizz3 ELISA kitRetn ELISA kitFizz3 ELISA kit |
| Specificity | This kit recognizes Mouse RETN in samples. No significant cross-reactivity or interference between Mouse RETN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-108 | 99 |
| EDTA plasma (n=8) | 91-103 | 97 |
| Cell culture media (n=8) | 92-105 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/ml) | 45.73 | 101.25 | 388.48 | 43.23 | 104.27 | 360.81 |
| Standard deviation | 2.83 | 4.36 | 14.96 | 2.32 | 5.89 | 16.92 |
| CV (%) | 6.18 | 4.31 | 3.85 | 5.37 | 5.65 | 4.69 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance