Myelin Associated Glycoprotein (MAG) (PE)

Cat# M9757-50-PE-100ul

Size : 100ul

Marca : US Biological

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M9757-50-PE Myelin Associated Glycoprotein (MAG) (PE)

Clone Type
Polyclonal
Host
mouse
Source
chicken
Isotype
IgG1
Grade
Affinity Purified
Applications
FLISA IC IHC WB
Crossreactivity
Bo Ch Fr Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Myelin-associated glycoprotein (MAG),a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100kD, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in the inhibition of nerve regeneration in the CNS.||Applications: |Suitable for use in FLISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Useful for myelinogenesis and multiple sclerosis studies. Other applications not tested.||Recommended Dilutions:|Western Blot: non-reducing conditions only|Immunohistochemistry: Frozen and Paraffin|Immunocytochemistry: methanol fixation required. |1. Oligodendrocytes are cultivated on slides and fixed with methanol for 10 minutes at-20°C in the middle log-phase. |2. After blocking the nonspecific binding sites with 5% FCS the cells are incubated with the properly diluted M9757-50 in a humid chamber for one hour at RT.|3. Afterwards cells are washed and incubated with anti-Mouse IgG-FITC. The slides are washed and covered appropriately|Optimal dilutions to be determined by the researcher.||Positive Control:|Rat hippocampus tissue, rat brain lysate, PC12 cells||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 2Q144 (513)|Host: mouse|Source: chicken|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein A affinity chromatography.|Immunogen: L2 epitope carrying glycoprotein extract of 1–2 day old chicken brain. |Specificity: Recognizes chicken Myelin Associated Glycoprotein at ~100kD. Species Crossreactivity: human, rat, mouse, bovine and frog.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
L2 epitope carrying glycoprotein extract of 1–2 day old chicken brain.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes chicken Myelin Associated Glycoprotein at ~100kD. Species Crossreactivity: human, rat, mouse, bovine and frog.
References
1. Trillo-Pazos, G., et al. (2004). Neuropathol. Appl. Neurobiol. 30(2):136-147. 2. Goolsby, J., et al. (2003). PNAS. 100:14926- 14931. 3. Ellezam, B., et al. (2003). Neurobiol Disease. 12:1-10. 4. Pang, Y., et al. (2000). J. Neurosci. Res. 62(4):510-520. 5. Collins, BE., et al. (1997). J Biol Chem. 272(27):16889-16895.