- More Files
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
- Specifications
Product Description
NADP+/NADPH Assay Kit is a ultrasensitive colorimetric determination of NADP+/NADPH at 565 nm.
Detection Method
Colorimetric
Calibration Range
0.1 to 10 uM
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store the kit at -20°C.
- Applications
Functional Study
- Publication Reference
- Mitochondrial serine catabolism safeguards maintenance of the hematopoietic stem cell pool in homeostasis and injury.
Shivani Rana, Leyla Nasr, Daniel Chang, Josephine Axis, Kurt Amsler.
Am J Physiol Cell Physiol. 2024 Oct 1;327(4):C913-C928. doi: 10.1152/ajpcell.00534.2023. Epub 2024 A 2024 Oct; 327(4):C913.
Application：WB, Dog, MDCK Type II cell.
- Salvage therapy expands highly cytotoxic and metabolically fit resilient CD8+ T cells via ME1 up-regulation.
Joanina K Gicobi, Zhiming Mao, Grace DeFranco, Jacob B Hirdler, Ying Li, Vianca V Vianzon, Emilia R Dellacecca, Michelle A Hsu, Whitney Barham, Yiyi Yan, Aaron S Mansfield, Yi Lin, Xiaosheng Wu, Taro Hitosugi, Dawn Owen, Michael P Grams, Jacob J Orme, Fabrice Lucien, Hu Zeng, Sean S Park, Haidong Dong.
Science Advances 2023 Nov; 9(46):eadi2414.
Application：Quant, Hunan, T cells.
- Cholesterol confers ferroptosis resistance onto myeloid-biased hematopoietic stem cells and prevents irradiation-induced myelosuppression.
Chaonan Liu, Weinian Liao, Jun Chen, Kuan Yu, Yiding Wu, Shuzhen Zhang, Mo Chen, Fang Chen, Song Wang, Tianmin Cheng, Junping Wang, Changhong Du.
Redox Biology 2023 Mar; 62:102661.
Application：Func, Mouse, Mouse hematopoietic stem cells (HSCs) .
- Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.
Changzheng Li, Binghuo Wu, Yishan Li, Jie Chen, Zhitao Ye, Xiaobin Tian, Jin Wang, Xi Xu, Shuai Pan, Yucan Zheng, Xiongwei Cai, Linjia Jiang, Meng Zhao.
Cell Stem Cell 2022 Jul; 29(7):1119.
Application：Func, Mouse, Mouse hematopoietic stem cells (HSCs).
- Thiol-specific Oxidant Diamide Downregulates whiA Gene of Corynebacterium glutamicum, thereby Suppressing Cell Division and Metabolism.
Haeri Jeong, Jae-Hyun Lee, Younhee Kim, Heung-Shick Lee.
Research in Microbiology 2020 Dec; 171(8):331.
Application：Func, Bacteria, Bacterial strains.
- Glycogen metabolism regulates macrophage-mediated acute inflammatory responses.
Ma J, Wei K, Liu J, Tang K, Zhang H, Zhu L, Chen J, Li F, Xu P, Chen J, Liu J, Fang H, Tang L, Wang D, Zeng L, Sun W, Xie J, Liu Y, Huang B.
Nature Communications 2020 Apr; 11(1):1769.
Application：Func, Mouse, BMDMs.
- The osnR gene of Corynebacterium glutamicum plays a negative regulatory role in oxidative stress responses.
Jeong H, Kim Y, Lee HS.
Journal of Industrial Microbiology & Biotechnology 2019 Feb; 46(2):241.
Application：Func, Corynebacterium glutamicum, Intracellular nucleotides.
- PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis.
Yang Q, Xu J, Ma Q, Liu Z, Sudhahar V, Cao Y, Wang L, Zeng X, Zhou Y, Zhang M, Xu Y, Wang Y, Weintraub NL, Zhang C, Fukai T, Wu C, Huang L, Han Z, Wang T, Fulton DJ, Hong M, Huo Y.
Nature Communications 2018 Nov; 9(1):4667.
Application：Func, Human, Supernatants collected from HUVECs culture medium.
- Restoring oxidant signaling suppresses proarthritogenic T cell effector functions in rheumatoid arthritis.
Yang Z, Shen Y, Oishi H, Matteson EL, Tian L, Goronzy JJ, Weyand CM.
Science Translational Medicine 2016 Mar; 8(331):331ra38.
Application：Quan, Human, CD4+CD45RO− T cells.
- Resveratrol Protects and Restores Endothelium-Dependent Relaxation in Hypercholesterolemic Rabbit Corpus Cavernosum.
Murat N, Korhan P, Kizer O, Evcim S, Kefi A, Demir Ö, Gidener S, Atabey N, Esen AA.
The Journal of Sexual Medicine 2016 Jan; 13(1):12.
Application：Func, Rabbit, Corpus cavernosum.
- GSTO1-1 modulates metabolism in macrophages activated through the LPS and TLR4 pathway.
Menon D, Coll R, O'Neill LA, Board PG.
Journal of Cell Science 2015 May; 128(10):1982.
Application：Func, Mouse, J774.1A cells.
- The Transcription Factor CREB Enhances Interleukin-17A Production and Inflammation in a Mouse Model of Atherosclerosis.
Kotla S, Singh NK, Heckle MR, Tigyi GJ, Rao GN.
Science Signaling 2013 Sep; 6(293):ra83.
Application：Quant, Mouse, Mouse macrophage.
- Phosphofructokinase deficiency impairs ATP generation, autophagy, and redox balancein rheumatoid arthritis T cells.
Yang Z, Fujii H, Mohan SV, Goronzy JJ, Weyand CM.
The Journal of Experimental Medicine 2013 Sep; 210(10):2119.
Application：Func, Human, T cell extracted.
- Mitochondrial serine catabolism safeguards maintenance of the hematopoietic stem cell pool in homeostasis and injury.