Daffodil lectin exists as a dimeric protein composed of two subunits of 13,000D devoid of attached carbohydrate. Below pH 5.0 and above pH 9.0 NPL dissociates into monomers. This lectin has a specificity toward alpha linked mannose, preferring polymannose structures containing (a-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA and PSA, NPL does not bind glucose. Staining of the cytoplasmic membrane of cells with NPL after short-term acid treatment can indicate those cells undergoing apoptosis. Detection of apoptotic cells using NPL binding is a highly sensitive and reliable tool for studying cell death. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation.||Applications: |Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Active Conjugate:|~99%||Lectin Biotinylated:|~99%||Inhibiting Sugar: |400mM alpha-methylmannoside||Sugar Specificity: |Mannose||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.