Neurofilament M,H 160-200kD (NF-M+H) (FITC)

Neurofilaments are intermediate (10–12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD), and NF-H (200kD). The neurofilaments are long helical proteins, which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, phosphorylation likely mediates neurofilament dynamics. However, how O-linked N-acetylglucosamine residues function to modify neurofilament function remains unclear. ||Applications:|Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. Other applications not tested. ||Recommended Dilution:|ELISA: 0.1–0.5ug/ml |Immunohistochemistry (Bouin’s and alcohol-fixed, paraffin-embedded sections and frozen sections): 5-10ug/ml. To stain, incubate 30–60 minutes at RT or overnight at 4°C. |Western Blot: 0.5-1ug/ml|Immunoprecipitation: 2-5ug|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.