This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Working Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/20,000.
Reactivity
Bovine
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Nucleoside phosphorylase isolated and purified from calf spleen. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity
Nucleoside Phosphorylase from Calf spleen. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Buffer
PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized hyperimmune IgG fraction
Reconstitution Method
Restore by adding 1.0 ml of sterile distilled water
Concentration
lot specific
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation
Unconjugated
Storage
Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. This enzyme, together with adenosine deaminase (ADA) serves a key role in purine catabolism. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
