Application
| WB, E |
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Primary Accession | P08183 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 141479 Da |
Antigen Region | 647-677 aa |
Gene ID | 5243 |
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Other Names | Multidrug resistance protein 1, ATP-binding cassette sub-family B member 1, P-glycoprotein 1, CD243, ABCB1, MDR1, PGY1 |
Target/Specificity | This P-Glycoprotein (ABCB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 647-677 amino acids from the Central region of human P-Glycoprotein (ABCB1). |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | P-Glycoprotein (ABCB1) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ABCB1 (HGNC:40) |
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Synonyms | MDR1, PGY1 |
Function | Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D- glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug- resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218). |
Cellular Location | Cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441} Apical cell membrane. Cytoplasm Note=ABCB1 localization is influenced by C1orf115 expression levels (plasma membrane versus cytoplasm). Localized to the apical membrane of enterocytes (PubMed:28408210). |
Tissue Location | Expressed in small intestine (PubMed:28408210). Expressed in liver, kidney and brain. |