PS100010 | pCMV6-AC-GFP Mammalian Expression Vector Clinisciences

Specifications
Product Data
C-Tag	TurboGFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Expression Promoter	CMV
Features	ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal tGFP tag. TurboGFP (excitation/ emission max = 482/ 502 nm) is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboGFP Such clones are the best for monitoring protein expression, localization, translocation and co-localization with other protein(s). If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal GFP-tagging vector, PS100019. V2_F Primer can be used to sequence the target sequence after cloning. AC_GFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping	Ambient

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS for PS100010

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

pCMV6-AC-GFP Mammalian Expression Vector