• Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDHA1.

    Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding S293 human PDHA1.

    Host

    Rabbit

    Reactivity

    Human

    Specificity

    This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein. It is recommended that BSA rather than non-fat milk be used in membrane blocking and antibody dilution. This antibody is useful for Immunocytochemistry/immunofluorescence and Western blot, where a band is seen ~43 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

    Form

    Liquid

    Conjugation

    DyLight 650

    Purification

    Immunogen affinity purification

    Recommend Usage

    Immunocytochemistry/Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 50 mM sodium borate (0.05% sodium azide).

    Storage Instruction

    Store at 4°C in the dark.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Immunocytochemistry

    Immunofluorescence

  • Gene Info — PDHA1

    Entrez GeneID

    5160

    Protein Accession#

    P08559

    Gene Name

    PDHA1

    Gene Alias

    PDHA, PDHCE1A, PHE1A

    Gene Description

    pyruvate dehydrogenase (lipoamide) alpha 1

    Omim ID

    300502 308930 312170

    Gene Ontology

    Hyperlink

    Gene Summary

    The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM

    Other Designations

    OTTHUMP00000023015|pyruvate dehydrogenase E1 alpha subunit

  • Interactomes
  • Pathways