Peroxidase BioAssay™ Kit (Colorimetric/Fluorimetric)

Peroxidases (EC number 1.11.1.x) catalyze the following oxidation reduction reactions:|ROOR' + electron donor (2 e-) + 2H+ › ROH + R'OH|For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as by products during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi and bacteria (cytochrome-c peroxidase like enzymes such a horseradish peroxidase).||Intended Use:|Quantitative colorimetric/fluorimetric Peroxidase determinations||Range:|colorimetric assays 4-1000IU/L, fluorimetric assays 0.8-25IU/L||Test Principle:|This Peroxidase BioAssay™ Kit uses H2O2 and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (lexc = 530nm, lem = 590nm) is a direct measure of the enzyme activity.||Kit Components:|Assay Buffer (pH 7.0), 1x20ml |Dye Reagent, 1x60ul|Stabilized H2O2, 3%, 1x100ul |Stop Reagent, 1x12ml|Calibrator, 1x5ml (equivalent to 1000IU/L)||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.