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> pGFP-V-RS shRNA Vector

pGFP-V-RS shRNA Vector

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Cat# TR30007

Size : 5ug

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pGFP-V-RS shRNA Vector

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SKU
TR30007
HuSH shRNA GFP Retroviral Cloning Vector (pGFP-V-RS Vector), 5 ug
Specifications
Product Data
Control Type Negative
Fluorescent Label GFP
Viral Packaging Retroviral
Features
  • The HuSH pGFP-V-RS plasmid vector contains both 5' and 3' LTRs of Moloney murine leukemia virus (MMLV) that flank the puromycin marker and the U6-shRNA expression cassette. Upon transient transfection of the plasmids into a packaging cell line, replication deficient viruses can be obtained and used to infect target cells. The puromycin-N-acetyl transferase gene and Kanamycin gene provide selection of antibiotics puromycin and kanamycin, respectively. There is an integrated turboGFP element driven by a cMV promoter to readily verify transfection efficiency.
  • U6_F Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Components 1 vial of 5 ug lyophilized pGFP-V-RS shRNA cloning vector
Quality Control This vector was sequence verified and functionally validated.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.

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