Phaseolus vulgaris (PHA-E) (FITC)

Phaseolus vulgaris Lectin (PHA-E) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers.||Source:|Kidney bean||Blood Specificity:|A (-SA)||Sugar Specificity:|Complex N-Glycans||Inhibitory Carbohydrate:|Bovine Thyroglobulin, Acetic Acid||Fluorescence:|Green||Divalent Ions:|Ca++, Mn++||Excitation:|495nm||Emission:|515nm||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.