Abx098112-5ml | Protein G Resin Clinisciences

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Datasheet SDS

Protein G Resin is an affinity chromatography resin with high binding capacity for IgG. Protein G Resin is suitable for purification of monoclonal antibodies, polyclonal antibodies and immunology complexes, such as IP and Co-IP. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose.

Specifications:

Resin: Cross-linked 4% agarose
Ligand: Recombinant Protein G
Shape: Sphere
Pore Size: 90 µm (45-165)
Support Density: 3 mg Protein G/ml wet gel
Binding Capacity: 20-25 mg h-IgG/ml wet gel
Maximum Flow Rate (25 °C): 300 cm/h
Recommended Flow Rate: < 150 cm/h
Highest Resisistance of Atmospheric Pressure: 0.3 MPa
pH Stability: 3-10

Affinity of Protein A/G for IgG types:

Source	IgG Subtype	Affinity for Protein A	Affinity for Protein G
Human	IgG₁	++++	++++
Human	IgG₂	++++	++++
Human	IgG₃	-	++++
Human	IgG₄	++++	++++
Mouse	IgG₁	+	++++
Mouse	IgG_2a	++++	++++
Mouse	IgG_2b	+++	+++
Mouse	IgG₃	++	+++
Rabbit	IgG	++++	+++
Goat	IgG	-	++
Horse	IgG	++	++++
Dog	IgG	++	+
Cow	IgG	++	++++
Pig	IgG	+++	+++
Monkey	IgG	++++	++++

Target	Protein G Resin
Storage	Store at 2-8 °C (with 20% ethanol) for up to 2 years.
Buffer	Note: Buffers are not included with this product. Equilibration Buffer: 20 mM PBS, 150 mM KCl, pH 7.0 Elution Buffer: 20 mM citric acid, pH 3.0-4.0; or 100 mM glycine, pH 3.0; or 20 mM sodium acetate, pH 3.0-4.0 Neutralisation Buffer: 1 M Tris-HCl, pH 9.0
Availability	Shipped within 10-20 working days.
Note	This product is for research use only.
Directions for use	Preparing the Protein G purification column: Thoroughly resuspend the protein G resin to achieve a homogeneous suspension of the resin in 20% ethanol storage buffer. Immediately transfer the resin into a purification column. Ensure that the bottom of the column is plugged with a stopper. Close the value of the column and allow the resin to settle. Equilibrate the column with 5-10 bed volume of equilibration buffer. Preparing samples: To avoid blocking the column, samples should be centrifuged and filtered through a 0.45 µm filter before loading. Loading samples and washing: Load samples and wash with 5-10 bed volume of equilibration buffer, and collect the flow-through in a tube Elute: Elute antibodies with elution buffer. Collect the elution containing the target immunoglobulin and immediately neutralise to pH > 7.0 with neutralisation buffer. The elution conditions are closely related with binding strength and stability of antibody. When necessary, optimise the elution buffer. Regeneration of Protein G resin: Either: Wash the column/resin with 3-5 bed volume of 0.1 M citric acid, or 0.1 M citric acid with 20% ethanol, and then 5 bed volume of PBS (pH 7.0); or 3-5 bed volume of 0.05 M NaOH with 1 M NaCl, or 6 M GuHCl, and then 5 bed volume of deionised water. Store the column/resin in 20% ethanol.

Research Articles on Protein G Resin

Protein G Resin

Cat# abx098112-5ml

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