Protein Phosphatase 2, methylated (formerly 2A), Catalytic Subunit, alpha Isoform (PP2A-alpha, PP2Ac, PP2CA, PP2Calpha, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform) (FITC)
Cat# P9107-34R5-FITC-100ul
Size : 100ul
Marca : US Biological
P9107-34R5-FITC Rabbit Anti-Protein Phosphatase 2, methylated (formerly 2A), Catalytic Subunit, alpha Isoform (PP2A-alpha, PP2Ac, PP2CA, PP2Calpha, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform) (FITC)
Clone Type
PolyclonalHost
mouseSource
humanSwiss Prot
P67775Isotype
IgG1,kGrade
Affinity PurifiedApplications
WBCrossreactivity
Hu Mo YeAccession #
NM_004156Shipping Temp
Blue IceStorage Temp
-20°CType 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the a subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.||Applications: |Suitable for use in Western Blot. Other applications not tested.||Recommended Dilution:|Western Blot: 1-5ug/ml. |Optimal dilutions to be determined by the researcher.||Positive Control:|Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.