Proteinase K from Tritirachium album min. 30 mAnson-U/mg
Cat# 33752.02
Size : 100MG
Marca : SERVA Electrophoresis
EC 3.4.21.14 • Mr ca. 28 000 • CAS [39450-01-6]
Serin protease with very broad range of action: cleaves peptide bonds at the carboxylic side of aliphatic, aromatic, and hydrophobic amino acids. Suitable for the isolation of DNA and RNA (1, 3).
Unit definition: 1 mAnson unit is defined as the amount of enzyme that liberates folin-positive amino acids and peptides, corresponding to 1 µmol tyrosine per minute at 37 °C and pH 7.4 using urea-denatured hemoglobin as substrate (4).
Inhibitors for proteinase: AEBSF (cat. no. 12745), (PEFABLOC® SC (cat. no. 31682) and PMSF (cat. no. 32395)
Extraneous activities: DNases and RNases not detectable
References:
- Ebeling, W. et al.(1974) Eur. J. Biochem. 47, 91-7
- Lin, Y. et al.(1969) J. Biol. Chem. 244, 789-93
- Sambrook, Fritsch, Maniatis (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press (B.16, 1.61)
- Anson, M.L. (1938) J. Gen. Physiol. 33, 79-89
DANGER |
Hazard Statements | H315 - H319 - H334 - H335 |
Precautions | P280 - P284 |
Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 |
EG-Index: 647-014-00-9 • EINECS: 254-457-8 • WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C