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> Pyruvic Acid Colorimetric Assay Kit

Pyruvic Acid Colorimetric Assay Kit

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Cat# E-BC-K130-M-96T

Size : 96T

Marca : Elabscience

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Telefono : +1 850 650 7790

Detection Principle Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm.
Sample Type Serum,Plasma,Tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (480-520 nm, optimum wavelength: 505 nm)
Research Area Amino Acids And Proteins ,  Mitochondria Function ,  Tricarboxylic Acid（TCA）Cycle ,  Cuproptosis ,  Energy Metabolism
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.003 μmol/mL
Detection Range 0.003-2.0 μmol/mL
Precision inter-assay CV: 3.6% | intra-assay CV: 2.3%
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K044-M L-Lactic Acid (LA) Colorimetric Assay Kit 96T , 48T
E-BC-K234-M Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method) 500Assays , 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Mouse serum 1
Mouse plasma 1
10% Mouse liver tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat heart tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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E-BC-K130-S-100
Pyruvic Acid Colorimetric Assay Kit
 100Assays