QuantiChrom™ Glucose Dehydrogenase Assay Kit
Application
- For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.
Key Features
- Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 200 U/L for 15 min reaction.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD565nm
Samples
- Biological samples (e.g. plasma, serum, tissue, and culture media)
Species
- All
Procedure
- 15 min
Size
- 100 tests
Detection Limit
- 0.5 U/L
Shelf Life
- 6 months
More Details
- GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donors with other acceptors. GDH participates in the pentose phosphate pathway. BioAssay Systems’ non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost