Recombinant Human Interleukin-33 (E. coli)

Biological Activity

Human Interleukin-33 (IL-33) is a proinflammatory cytokine that belongs to the IL-1 family. IL-33 plays a role in autoimmune disease. IL-33’s interaction with ST2 can drive allergic pathology and IL-33 has been reported to play a role in the development of rheumatoid arthritis and systemic lupus erythematosus. IL-33 is expressed in a variety of cells, including epithelial and endothelial cells, smooth muscle cells, macrophages and fibroblasts. The primary receptors for IL-33 are ST2 and IL-1 receptor accessory protein (IL-1RAcP), both of which belong to the IL-1 receptor family. IL-33 is localized to the nucleus of resting cells where it binds to chromatin in the H2A-H2B histone complex as a transcriptional suppressor. IL-33 is secreted by cells during injury which induces a T-helper 2 type inflammatory response.

ED50 < 0.5 ng/ml, measured by a cell proliferation assay using D10S cells, corresponding to a specific activity of > 2.0 × 10⁶ units/mg.

Recombinant Human Interleukin-33 (IL-33) expressed the target gene encoding Ser112-Thr270.

Accession: O95760

Endotoxin < 0.2 EU/µg

Apparent Molecular Weight: ~18.0 kDa, reducing conditions

Lyophilized after extensive dialysis against PBS.

Chemical Information

Form	Lyophilized powder
Solubility (25°C)	Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml.
Storage	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.

References

[1] Akira Matsuda, et al. Invest Ophthalmol Vis Sci . The role of interleukin-33 in chronic allergic conjunctivitis