Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Recombinant Proteinase K

Recombinant Proteinase K

Stampa Stampa
Entra

Cat# HY-P3150-50mg

Size : 50mg

Marca : MedChemExpress

Contatta il distributore locale :


Telefono : +1 850 650 7790

Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Recombinant Proteinase K Chemical Structure

Recombinant Proteinase K Chemical Structure

This product is a controlled substance and not for sale in your territory.

Description

Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations[1].

In Vitro

Proteinase K digestion[1]
1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.
2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 μl aliquot to get the final concentrations of 2, 4 and 8 μg/ml. Reactions are carried out at 37°C for 30 minutes.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Recombinant Proteinase K Related Antibodies
Appearance

Solid

Color

Off-white to light yellow

SMILES

[Recombinant Proteinase K]
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références

  • [1]. Sabine Gauczynski, et al. Recombinant human prion protein mutants huPrP D178N/M129 (FFI) and huPrP+9OR (fCJD) reveal proteinase K resistance. J Cell Sci. 2002 Nov 1;115(Pt 21):4025-36.  [Content Brief]

  • [1]. Sabine Gauczynski, et al. Recombinant human prion protein mutants huPrP D178N/M129 (FFI) and huPrP+9OR (fCJD) reveal proteinase K resistance. J Cell Sci. 2002 Nov 1;115(Pt 21):4025-36.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Recombinant Proteinase K Related Classifications

  • Others
  • Metabolic Enzyme/Protease
  • Ser/Thr Protease

  • Calculateur de molarité

  • Calculateur de dilution

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Recombinant Proteinase KSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesaromatic amino acidcarboxy-terminated peptide nucleic aciddigestionInhibitorinhibitorinhibit