Recombinant Proteinase K
Cat# HY-P3150-50mg
Size : 50mg
Marca : MedChemExpress
Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations.
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Recombinant Proteinase K Chemical Structure
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Description |
Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations[1]. |
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In Vitro |
Proteinase K digestion[1]1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 μl aliquot to get the final concentrations of 2, 4 and 8 μg/ml. Reactions are carried out at 37°C for 30 minutes. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
Appearance |
Solid |
Color |
Off-white to light yellow |
SMILES |
[Recombinant Proteinase K] |
Livraison | Room temperature in continental US; may vary elsewhere. |
Stockage |
Please store the product under the recommended conditions in the Certificate of Analysis. |
Pureté et documentation |
Purity: ≥95.0% |
Références |
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Recombinant Proteinase K Related Classifications
- Others
- Metabolic Enzyme/Protease
- Ser/Thr Protease
Calculateur de molarité
Calculateur de dilution
The molarity calculator equation
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Recombinant Proteinase KSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesaromatic amino acidcarboxy-terminated peptide nucleic aciddigestionInhibitorinhibitorinhibit