Reduced Glutathione (GSH) BioAssay™ Kit

Glutathione (GSH) is a kind of low molecular scavenger, which can remove O2-, H2O2, LOOH. Reduced GSH is a small molecule peptide which composed of glutamic acid, glycine and cysteine, and it is the main thiol compound of non-protein in the organization. GSH is the substrate of GPX and GST which is indispensable for decomposing hydrogen peroxide of these two enzymes, and it can stabilize the enzyme containing thiol and prevent hemoglobin and other auxiliary factors from the oxidative damage. Recently, it is proved that GSH is also involved in the recovery of VE to the reduction state. Recently, it is proved that GSH is also involved in the recovery of VE to the reduction state. When lacking or depletion of GSH, it may cause producing toxic effects or increasing the toxic effects of many chemicals or environmental factors. It may be related to the increase of oxidative damage, so the amount of GSH is a vital factor to measure the body's antioxidant ability. GSH plays an important role in the researches process of prevention, recovery and treatment of atherosclerosis, coronary heart disease, anti-aging, anti-tumor, prevention, prevention of Alzheimer's disease and other diseases.||Sample Type:|Animal serum, plasma, tissue, whole blood, red blood cells, culture cells, culture medium and other samples.||Intended Use:|This kit can be used to measure the GSH content in animal serum, plasma, tissue, whole blood, red blood cells, culture cells, culture medium and other samples.||Test Principle:|Reduced GSH can react with DNTB and form yellow complex, which can be detected by colorimetric assay at 405nm and calculate the reduced GSH content.||Kit Components (see Protocol for final kit quantities):|Reagent 1|Precipitant, 1x20mL||Reagent 2|Buffer, 1x20mL||Reagent 3|Chromogenic agent, 1x5mL||Reagent 4|GSH standard, powder, 3.07mg × 3vial|Concentrated standard diluent (10×), 1x10ml||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.