Revitablot Western Blot Stripping Buffer

Western Blot comparison of Revitablot™ Western Blot Stripping Buffer using Fluorescent labeled Primary Antibodies. Lane 1: 7.5 µg HCT-116 cell lysates. Lane 2: 22.5 µg HCT-116 cell lysates. Lane 3: 15.0 µg HCT-116 cell lysates. Block: 5% BLOTTO for 1 hr at room temperature. Blot A first probed with: Primary antibody: Mouse anti-AKT pS473 at a dilution of 1:1000 overnight at 4°C. Imaging: 650nm. Membranes were then incubated with Revitablot™ 5 minutes at room temperature, followed by re-blocking with 5% BLOTTO for 2 hours at room temperature. Blot B (Blot A reprobed) with: Primary antibody: Rabbit anti-beta actin DyLight 488 Conjugated at a dilution of 1:1000 for 4 hours at room temperature. Imaging: 488nm and 650nm.

Western Blot comparison of Revitablot™ Western Blot Stripping Buffer. Lane 1: 70 µg HeLa whole cell lysates. Lane 2: 10 µg HeLa whole cell lysates. Blot A first probed with: Primary antibody: Rabbit Anti-STAT5 (p/n orb345603) at a dilution of 1:1000 overnight at 4°C. Secondary antibody: HRP Goat anti-rabbit (p/n orb347654) at 1:10000 dilution. Membranes were then incubated with Revitablot and a competitor's product for 20 minutes at room temperature, followed by re-blocking with 5% BLOTTO for 2 hours at room temperature. Blot B (Blot A reprobed) with: Primary antibody: Rabbit anti-tubulin (p/n orb345510) at a dilution of 1:1000 overnight at 4°C. Secondary antibody: HRP Goat anti-rabbit (p/n orb347654) at 1:10000 dilution.