RIPA Lysis Buffer System
Cat# sc-24948A
Size : 500ml
Marca : Santa Cruz Biotechnology
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RIPA Lysis Buffer System: sc-24948
RIPA Lysis Buffer System
Noms alternatifs:
RIPA Lysis Buffer System also known as RIPA (Radioimmunoprecipitation) Lysis Buffer System
Application:
RIPA Lysis Buffer System® is a high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
* Consulter le Certificat d'Analyses pour les données spécifiques à un lot (incluant la teneur en eau).
ACCÈS RAPIDE AUX LIENS
Informations pour la commande
Références bibliographiques
Description
Information Technique
Données de Sécurité
SDS & Certificat d’Analyses
The RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer System is extensively used in the field of biochemistry and molecular biology for the lysis and solubilization of cells and tissues. The buffer is designed to efficiently disrupt cell membranes while solubilizing cellular proteins, making it an ideal choice for protein extraction for subsequent analyses such as Western blotting and immunoprecipitation. RIPA buffer contains a mixture of three detergents—sodium deoxycholate, NP-40, and SDS. This combination is particularly effective at solubilizing a wide range of protein classes, making it suitable for comprehensive studies of cellular protein compositions. The sodium deoxycholate and NP-40 are non-ionic and ionic detergents respectively, that disrupt more of the lipid-lipid and lipid-protein interactions, whereas SDS provides a strong anionic environment that denatures proteins, ensuring their solubility. The effectiveness of RIPA buffer is further enhanced by the presence of buffering agents and additives such as Tris, which maintains a consistent pH, and sodium chloride, which provides ionic strength that can help in protein stabilization. Optional components like EDTA can be added to chelate divalent cations and prevent protease degradation of the target proteins. In scientific research, RIPA buffer is valued for its robustness and versatility in extracting proteins under conditions that maintain most post-translational modifications and protein-protein interactions. This makes it a critical tool in proteomics and other cellular biology studies where detailed understanding of protein interactions and functions is essential.
References:
- Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers. | Ngoka, LC. 2008. Proteome Sci. 6: 30. PMID: 18950484
- Analysis of pp60c-src tyrosine kinase activity and phosphotyrosyl phosphatase activity in human colon carcinoma and normal human colon mucosal cells. | DeSeau, V., et al. 1987. J Cell Biochem. 35: 113-28. PMID: 2448318
- Analysis of pp60c-src in human colon carcinoma and normal human colon mucosal cells. | Bolen, JB., et al. 1987. Oncogene Res. 1: 149-68. PMID: 2453014
- Monitoring Autophagic Flux by Using Lysosomal Inhibitors and Western Blotting of Endogenous MAP1LC3B. | Chittaranjan, S., et al. 2015. Cold Spring Harb Protoc. 2015: 743-50. PMID: 26240408
- Nitric Oxide Is Involved in Activation of Toll-Like Receptor 4 Signaling through Tyrosine Nitration of Src Homology Protein Tyrosine Phosphatase 2 in Murine Dextran Sulfate-Induced Colitis. | Tun, X., et al. 2018. Biol Pharm Bull. 41: 1843-1852. PMID: 30504685
- Interaction of the mTERT telomerase catalytic subunit with the c-Abl tyrosine kinase in mouse granulosa cells. | Yaba, A., et al. 2020. J Recept Signal Transduct Res. 40: 365-373. PMID: 32131672
- Downregulation of lncRNA PVT1 inhibits proliferation and migration of mesothelioma cells by targeting FOXM1. | Fujii, Y., et al. 2022. Oncol Rep. 47: PMID: 34859258
- AMP-activated Protein Kinase Activation Suppresses Protein Synthesis and mTORC1 Signaling in Chick Myotube Cultures. | Nakashima, K. and Ishida, A. 2022. J Poult Sci. 59: 81-85. PMID: 35125916
- A ts T mutant of Schmidt Ruppin strain of Rous sarcoma virus restricted at 39.5 degrees C for the morphological transformation and the tumorigenicity of chicken embryo fibroblasts. | Poirier, F., et al. 1982. Int J Cancer. 29: 69-76. PMID: 6277805
- Interaction of the mTERT telomerase catalytic subunit with the c-Abl tyrosine kinase in mouse granulosa cells. | Yaba and Aylin, et al. 2020. Journal of Receptors and Signal Transduction. 40.4: 365-373.
Utilisation :
1. Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.
2. Use 3 ml complete RIPA per gram of tissue
- OR 1 ml complete RIPA per 2.0x107 cells in suspension
- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
État Physique :
Liquid
STOCKAGE :
Store at -20° C
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
Télécharger la Fiche de Sécurité/ SDS (MSDS)
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SAMPLE Certificate of Analysis (COA)
RIPA Lysis Buffer System SAMPLE Certificate of AnalysisCERTIFICAT D'ANALYSE
Numéro de catalogue
Numéro de Lot
Envoyer
SAMPLE Certificate of Analysis (COA)
Catalog Number:sc-24948
Lot Number:SAMPLE
Product Name:RIPA Lysis Buffer System
Test | Specification | Results |
---|---|---|
Appearance | RIPA Lysis Buffer: Clear, water white liquidNa-Ortho: Clear, colorless liquidPMSF: Clear, colorlessPIC: Slightly yellow liquid | |
pH | RIPA Lysis Buffer: 7.355Na-Ortho: 10.004 | |
Sterility | RIPA Lysis Buffer: Filtered with 0.2 um filterNa-Ortho: Filtered with 0.22 um filter |
TEST CONDITIONS
Exp. Date: 7/2/2027
Informations pour la commande
Nom du produit | Ref. Catalogue | COND. | Prix HT | QTÉ | Favoris | |
RIPA Lysis Buffer System, 50 ml | sc-24948 | 50 ml | .00 | |||
RIPA Lysis Buffer System, 500 ml | sc-24948A | 500 ml | .00 |