Sambucus nigra (SNA I)

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. ||Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.||SNA-I is specific for alpha 2,6 linked sialic acid residues||Carbohydrate Specificity:|NANA a(2,6)GalNAc>GalNAc=lactose>galactose NANA a(2,6) galactose||Inhibitory Specificity:|High concentration Lactose>b-Galactose||Activity:|Less than 10ug/ml will exhibit agglutination with 3% type O human red blood cells in 10 minutes at RT. The lectin will also react with neuraminidase treated erythrocytes.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.