Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344]

Stampa

Cat# GTX635679-25ul

Size : 25ul

Contatta il distributore locale :

Telefono : +1 850 650 7790

Marca : GeneTex

Richiedere ulteriori informazioni

Contatta il distributore locale :

Telefono : +1 850 650 7790

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635679

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL344
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl, 25 μl

Datasheet File

View product citations for antibody GTX635679 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:500-1:1000
IHC-Fr	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : IHC-P Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes. Sandwich ELISA Capture : GTX635679, Detection : GTX635678 / GTX635688 / GTX635686-01. Please notice that GTX635688 / GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by protein A.

Conjugation

Unconjugated

RRID

AB_2888553

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

DATA IMAGES

GTX635679 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635679 IHC-P Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

GTX635679 IHC-P Image

GTX635679 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.074 nM

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

GTX635679 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.

GTX635679 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635679 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635679 ELISA Image

GTX635679 WB Image

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 virus (Wild type or B.1.617.2 delta variant) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635679 IHC-P Image

The data was published in the 2022 in Sci Rep. PMID: 35264719

GTX635679 IHC-P Image

The data was published in the 2022 in Sci Rep. PMID: 35264719

Application Reference

Elsharkawy A et al. Front Microbiol 2024; 15 : 1466980 Omicron XBB.1.5 subvariant causes severe pulmonary disease in K18-hACE-2 mice.

Qiao H et al. J Med Virol 2024; 96 (5) : e29671 SARS-CoV-2 induces blood-brain barrier and choroid plexus barrier impairments and vascular inflammation in mice.

Yu M et al. J Virol 2024; 98 (6) : e0162423 Multiple E3 ligases act as antiviral factors against SARS-CoV-2 via inducing the ubiquitination and degradation of ORF9b.

T Honda et al. Vaccines 2024; 12 (7) : 794 Intranasally Inoculated SARS-CoV-2 Spike Protein Combined with Mucoadhesive Polymer Induces Broad and Long-Lasting Immunity

Application : IHC-P

ST Homma et al. Biomedicines 2024; 12 : 1443 Respiratory SARS-CoV-2 Infection Causes Skeletal Muscle Atrophy and Long-Lasting Energy Metabolism Suppression

Application : IHC-P

Submit a Reference

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] (GTX635679). Be the first to share your experience with this product.

Application Reference

Elsharkawy A et al. Front Microbiol 2024; 15:1466980 Omicron XBB.1.5 subvariant causes severe pulmonary disease in K18-hACE-2 mice.

Qiao H et al. J Med Virol 2024; 96 (5):e29671 SARS-CoV-2 induces blood-brain barrier and choroid plexus barrier impairments and vascular inflammation in mice.

Yu M et al. J Virol 2024; 98 (6):e0162423 Multiple E3 ligases act as antiviral factors against SARS-CoV-2 via inducing the ubiquitination and degradation of ORF9b.

T Honda et al. Vaccines 2024; 12 (7):794 Intranasally Inoculated SARS-CoV-2 Spike Protein Combined with Mucoadhesive Polymer Induces Broad and Long-Lasting Immunity

Application : IHC-P

ST Homma et al. Biomedicines 2024; 12:1443 Respiratory SARS-CoV-2 Infection Causes Skeletal Muscle Atrophy and Long-Lasting Energy Metabolism Suppression

Application : IHC-P

K Takada et al. J Virol 2024;:e0178423 Genes involved in the limited spread of SARS-CoV-2 in the lower respiratory airways of hamsters may be associated with adaptive evolution

Application : IHC-P

Tsumita T et al. Aging Cell 2024; 23 (2):e14050 Viral uptake and pathophysiology of the lung endothelial cells in age-associated severe SARS-CoV-2 infection models.

Application : IHC-P

Imbiakha B et al. Sci Adv 2024; 10 (1):eadg5461 Adaptive immune cells are necessary for SARS-CoV-2-induced pathology.

Application : IHC-P

L Zhang et al. mBio 2024;:e0307123 CRL4B E3 ligase recruited by PRPF19 inhibits SARSCoV-2 infection by targeting ORF6 for ubiquitin-dependent degradation

Application : WB, IHC-P

Gupte SA et al. Br J Pharmacol 2023; 180 (20):2677-2693 The severity of SARS-CoV-2 infection in K18-hACE2 mice is attenuated by a novel steroid-derivative in a gender-specific manner.

V Castro et al. ACS Nano 2023; 17 (22):22708-22721 Three-Dimensional Remodeling of SARS-CoV2-Infected Cells Revealed by Cryogenic Soft X-ray Tomography

Application : ICC/IF

Uemura K et al. Proc Natl Acad Sci U S A 2023; 120 (42):e2304139120 2-thiouridine is a broad-spectrum antiviral nucleoside analogue against positive-strand RNA viruses.

Application : ICC/IF

Imbiakha B et al. Sci Adv 2023; 9 (38):eadj1736 Age-dependent acquisition of pathogenicity by SARS-CoV-2 Omicron BA.5.

Application : IHC-P

P?rez-Vargas J et al. Emerg Microbes Infect 2023; 12 (2):2246594 A novel class of broad-spectrum active-site-directed 3C-like protease inhibitors with nanomolar antiviral activity against highly immune-evasive SARS-CoV-2 Omicron subvariants.

Application : IHC-P, ELISA

S Andreu et al. Front Microbiol 2023; 14:1185504 Dextran sulfate from Leuconostoc mesenteroides B512F exerts potent antiviral activity against SARS-CoV-2 in vitro and in vivo

Application : ICC/IF

Cloherty APM et al. Emerg Microbes Infect 2023; 12 (1):2195020 Berbamine suppresses intestinal SARS-CoV-2 infection via a BNIP3-dependent autophagy blockade.

Application : ICC/IF

Li Z et al. Signal Transduct Target Ther 2023; 8 (1):53 The E3 ligase RNF5 restricts SARS-CoV-2 replication by targeting its envelope protein for degradation.

Application : WB, IHC-P

Gon?alves R et al. iScience 2023; 26 (2):105972 SARS-CoV-2 variants induce distinct disease and impact in the bone marrow and thymus of mice.

Application : IHC-P

P?rez-Vargas J et al. Antiviral Res 2023; 209:105484 Discovery of lead natural products for developing pan-SARS-CoV-2 therapeutics.

Application : ICC/IF

Garcia G Jr et al. PLoS Biol 2022; 20 (11):e3001851 Hippo signaling pathway activation during SARS-CoV-2 infection contributes to host antiviral response.

Application : WB

Shapira T et al. Mol Biomed 2022; 3 (1):43 Inhibition of glycogen synthase kinase-3-beta (GSK3β) blocks nucleocapsid phosphorylation and SARS-CoV-2 replication.

Application : ICC/IF

Yaron TM et al. Sci Signal 2022; 15 (757):eabm0808 Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication.

Application : WB

Shimizu H et al. iScience 2022; 25 (11):105314 LIGHTHOUSE illuminates therapeutics for a variety of diseases including COVID-19.

Application : ICC/IF

Yamamoto Y et al. Int J Mol Sci 2022; 24 (1) Direct Inhibition of SARS-CoV-2 Spike Protein by Peracetic Acid.

Application : WB

Yamamoto Y et al. Int J Mol Sci 2022; 23 (24) SARS-CoV-2 Spike Protein Mutation at Cysteine-488 Impairs Its Golgi Localization and Intracellular S1/S2 Processing.

Application : WB

Chan JF et al. Cell Rep Med 2022; 3 (10):100774 A molecularly engineered, broad-spectrum anti-coronavirus lectin inhibits SARS-CoV-2 and MERS-CoV infection in?vivo.

Application : IHC-P

Ma H et al. Cell Res 2022; 32 (9):831-842 Hetero-bivalent nanobodies provide broad-spectrum protection against SARS-CoV-2 variants of concern including Omicron.

Application : ICC/IF

Tirosh Shapira et al. Nature 2022; 605 (7909):340-348 A? TMPRSS2 inhibitor acts as a pan-SARS-CoV-2 prophylactic and therapeutic.

Application : IHC-P

Lissenya B Argueta et al. iScience 2022; 25 (5):104223 Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy.

Application : ICC/IF, IHC-P

Naoya Kitamura et al. J Med Chem 2022; 65 (4):2848-2865 Expedited Approach toward the Rational Design of Noncovalent SARS-CoV-2 Main Protease Inhibitors.

Application : ICC/IF

Ravindra Gupta et al. Res Sq 2022; SARS-CoV-2 Omicron spike mediated immune escape and tropism shift.

Application : WB

Yasui F et al. Sci Rep 2022; 12 (1):4150 Infection with the SARS-CoV-2 B.1.351 variant is lethal in aged BALB/c mice.

Application : IHC-P

Meng B et al. Nature 2022; (Epub) Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts tropism and fusogenicity.

Application : WB

Suzuki R et al. Nature 2022; (Epub) Attenuated fusogenicity and pathogenicity of SARS-CoV-2 Omicron variant.

Application : WB

Wright T et al. ACS Appl Mater Interfaces 2022; 14 (1):49-56 Photodynamic and Contact Killing Polymeric Fabric Coating for Bacteria and SARS-CoV-2.

Huan Ma et al. J Virol 2021; Potent Neutralization of SARS-CoV-2 by Hetero-bivalent Alpaca Nanobodies Targeting the Spike Receptor-Binding Domain.

Application : ICC/IF

Lissenya B Argueta et al. bioRxiv 2021; SARS-CoV-2 Infects Syncytiotrophoblast and Activates Inflammatory Responses in the Placenta.

Application : ICC/IF, IHC-P

Chunlong Ma et al. ACS Cent Sci 2021; 7 (7):1245-1260 Discovery of SARS-CoV-2 Papain-like Protease Inhibitors through a Combination of High-Throughput Screening and a FlipGFP-Based Reporter Assay.

Application : ICC/IF

Mona Seifert et al. Elife 2021; 10 Inhibition of SARS-CoV-2 polymerase by nucleotide analogs from a single-molecule perspective.

Application : ICC/IF

Jiang W et al. Cell Rep 2021; 37 (11):110112 A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge.

Application : IHC-P

Conforti A et al. Mol Ther 2021; (Epub) COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models.

Application : IHC-P

Ohno M et al. Viruses 2021; 13 (11) Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2.

Application : IHC-P

Garc?a-Dorival I et al. Antiviral Res 2021; 194:105167 Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target.

Application : ICC/IF

Asaka MN et al. JCI Insight 2021; 6 (19) Highly susceptible SARS-CoV-2 model in CAG promoter-driven hACE2-transgenic mice.

Application : IHC-P

Tsuji K et al. J Toxicol Sci 2021; 46 (9):425-435 Development of alveolar and airway cells from human iPS cells: toward SARS-CoV-2 research and drug toxicity testing.

Application : ICC/IF

Sasaki M. et al. mBio. 2021;:e0141521 SARS-CoV-2 Bearing a Mutation at the S1/S2 Cleavage Site Exhibits Attenuated Virulence and Confers Protective Immunity.

Application : IHC-P

Sci Immunol 2021; 6 (59) Pharmacological activation of STING blocks SARS-CoV-2 infection.

Application : ICC/IF

Shapira T. et al. bioRxiv 2021; (Epub) A novel highly potent inhibitor of TMPRSS2-like proteases blocks SARS-CoV-2 variants of concern and is broadly protective against infection and mortality in mice.

Application : ICC/IF

Ruetalo N et al. mSphere 2021; 6 (1) Antibody Response against SARS-CoV-2 and Seasonal Coronaviruses in Nonhospitalized COVID-19 Patients.

Application : WB

Eric Song et al. J Exp Med 2021; 218 (3):e20202135 Neuroinvasion of SARS-CoV-2 in human and mouse brain.

Application : IHC-Fr

Sacco MD et al. Sci Adv 2020; 6 (50) Structure and inhibition of the SARS-CoV-2 main protease reveal strategy for developing dual inhibitors against M^pro and cathepsin L.

Application : ICC/IF

Song E et al. bioRxiv 2020; (Epub) Neuroinvasion of SARS-CoV-2 in human and mouse brain.

Application : IHC-Fr

Sacco MD et al. bioRxiv 2020; Structure and inhibition of the SARS-CoV-2 main protease reveals strategy for developing dual inhibitors against M^pro and cathepsin L.

Application : ICC/IF

Heaton BE et al. bioRxiv 2020; (Epub) SRSF protein kinases 1 and 2 are essential host factors for human coronaviruses including SARS-CoV-2.

Application : WB

Anand Ramani et al. EMBO J. 2020;:e106230 SARS-CoV-2 targets neurons of 3D human brain organoids.

Application : WB, ICC/IF

Eric Song et al. bioRxiv 2020; (Epub) Neuroinvasive potential of SARS-CoV-2 revealed in a human brain organoid model.

Application : IHC-Fr

SDS
PBS.pdf

Si può anche essere interessati nei seguenti prodotti:

Cat#

Descrizione

Cond.

Priced

GTX135357-25ul

SARS-CoV-2 (COVID-19) Nucleocapsid antibody

25ul

GTX635678-25ul

SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL249]

25ul

GTX635686-25ul

SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448]

25ul

SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344]

Cat# GTX635679-25ul

Contatta il distributore locale :

Marca : GeneTex

Contatta il distributore locale :

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

DATA IMAGES

GTX635679 ELISA Image

GTX635679 IHC-P Image

GTX635679 IHC-P Image

GTX635679 WB Image

GTX635679 ELISA Image

GTX635679 ELISA Image

GTX635679 WB Image

GTX635679 ELISA Image

GTX635679 IHC-P (cell pellet) Image

GTX635679 ELISA Image

GTX635679 ICC/IF Image

GTX635679 ELISA Image

GTX635679 WB Image

GTX635679 ELISA Image

GTX635679 IHC-P Image

GTX635679 IHC-P Image

Application Reference

REVIEW

Si può anche essere interessati nei seguenti prodotti: