SR Proteins (Non-snRNP Splicing Factor) (APC)

SR proteins are a highly conserved family of arginine/serine rich, spliceosome associated phosphoproteins essential for metazoan pre-mRNA splicing. SR proteins appear to act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role, because they can determine alternative splice site usage in vivo and in vitro-a particularly interesting example is the regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins. SR proteins appear to be recruited from nuclear “speckles”, in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus. ||The epitope detected by the antibody has been termed the “alternating arginine domain” and is composed almost exclusively of argnine alternating with glutamate and aspartate. Antibody targets are predominantly nuclear, nonnucleolar, and are localized to active sites of polymerase II transcription. Epitope is conserved in metazoans.||Applications: |Suitable for use in Immunofluorescence, Immunocytochemistry, FLISA and Western Blot. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Positive Controls: |HeLa cell, nuclear extracts||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.