Western Blot. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl of 1/40-1/80 diluted antibody to label 10e6 cells in 100 µl (Routinely tested on Rat thymocytes).
Reactivity
Guinea Pig, Mouse, Rabbit, Rat
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Rat Thy1 antigen.
Specificity
This antibody recognizes Rat and Mouse CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in Mice, clone MRC OX-7 reacts with Thy1.1 Mice e.g. AKR and FVB Mice, but not Thy1.2 Mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. This clone has been reported to induce glomerular nephritis in Wistar Rats.
Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (Mouse), immature B cells (Rat) and connective tissues.
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