Thy1 Mouse Monoclonal Antibody [Clone ID: OX-7]

Specifications

Product Data
Clone Name	OX-7
Applications	FC, IHC, IP, WB
Recommended Dilution	Western Blot. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl of 1/40-1/80 diluted antibody to label 10e6 cells in 100 µl (Routinely tested on Rat thymocytes).
Reactivities	Guinea Pig, Mouse, Rabbit, Rat
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Rat Thy1 antigen.
Specificity	This antibody recognizes Rat and Mouse CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in Rat but polymorphic in Mice, clone MRC OX-7 reacts with Thy1.1 Mice e.g. AKR and FVB Mice, but not Thy1.2 Mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. This clone has been reported to induce glomerular nephritis in Wistar Rats.
Formulation	PBS, pH 7.2 without preservatives State: Low Endotoxin State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage	Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	thymus cell antigen 1, theta
Database Link	Entrez Gene 21838 Mouse UniProt ID P01831
Background	Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (Mouse), immature B cells (Rat) and connective tissues.
Synonyms	Thy-1, THY1, CDw90
Note	Endotoxin Level: Less than 0.01 EU/µg
Reference Data