TLR4 Mouse Monoclonal Antibody [Clone ID: HTA125]
Specifications
Product Data | |
Clone Name | HTA125 |
Applications | FC, FN, IP, WB |
Recommended Dilution | Flow Cytometry: Use 10 μl of 1/10-1/25 diluted antibody to label 106 cells or 100 μl whole blood. Immunoprecipitation. Western Blot. Functional Assays. |
Reactivities | Canine, Guinea Pig, Human, Monkey, Porcine |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
Specificity | This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. |
Formulation | PBS State: Low Endotoxin State: Liquid purified IgG fraction Stabilizer: None Preservative: None |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | toll like receptor 4 |
Database Link | |
Background | TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. |
Synonyms | Toll-like receptor 4 |
Reference Data | |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway |
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