TLR4 Mouse Monoclonal Antibody [Clone ID: HTA125]

Specifications

Product Data
Clone Name	HTA125
Applications	FC, FN, IP, WB
Recommended Dilution	Flow Cytometry: Use 10 μl of 1/10-1/25 diluted antibody to label 106 cells or 100 μl whole blood. Immunoprecipitation. Western Blot. Functional Assays.
Reactivities	Canine, Guinea Pig, Human, Monkey, Porcine
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS.
Formulation	PBS State: Low Endotoxin State: Liquid purified IgG fraction Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	toll like receptor 4
Database Link	Entrez Gene 574360 MonkeyEntrez Gene 7099 Human UniProt ID O00206
Background	TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Synonyms	Toll-like receptor 4
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway