Transetta(DE3) Chemically Competent Cell is specifically designed from imported strain for chemical transformation of DNA. It is resistant to chloramphenicol(Cam^R) and permits a transformation efficiency of over 10⁷ cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype
F^- ompT hsdSB(r_B^-m_B^-) gal dcm lacY1(DE3)pRARE(argU, argW, ileX, glyT, leuW, proL)(Cam^R)

Features
• Transformation efficiency: >10⁷ cfu/μg (pUC19 DNA).
• Cam^R.
• tRNAs for 6 rare codons AUA, AGG, AGA, CUA, CCC, GGA. Enhance the expression level of proteins in the prokaryotic system.
• Control plasmid I (Amp⁺) is used for detection of expression function of cell. The protein size is about 25 kDa.

Storage
at -70℃ for six months. Do not store in liquid nitrogen.

Shipping
Dry ice (-70℃)

Wu J , Gao B , Zhu S ,et al. Single-point mutation-mediated local amphipathic adjustment dramatically enhances antibacterial activity of a fungal defensin[J]. Faseb Journal Official Publication of the Federation of American Societies for Experimental Biology, 2016, 30(7):2602.

Zhou C , Ma K , Gao R , et al. Regulation of mATG9 trafficking by Src- and ULK1-mediated phosphorylation in basal and starvation-induced autophagy[J]. Cell Research, 2017, 27(2):184-201.

Li J , Fu J , Chen Y , et al. The U6, Biogenesis-Like 1, Plays an Important Role in Maize Kernel and Seedling Development by Affecting the 3′ End Processing of U6 snRNA[J]. Molecular Plant, 2017, 10(3):470-482.

Wang Y , Shi M , Feng H , et al. Structural Insights into Non-canonical Ubiquitination Catalyzed by SidE[J]. Cell, 2018:S0092867418305166.

Wang J, Zhou L, Shi H , et al. A single transcription factor promotes both yield and immunity in rice.Science. 2018 ,361(6406):1026-1028.