TransScript^® II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent cross contamination.

Highlights

• Use TransScript® II Multiplex Probe One-Step Enzyme Mix UDG, 2×PerfectStart® Multiplex Probe One-Step Reaction Mix to efficiently synthesize RNA into first-strand cDNA for qPCR. It is easy to operate and reduces the chance of contamination during operation. 

• Using UDG enzyme and dUTP to effectively prevent cross-contamination of PCR products with accurate data. 

• High sensitivity, high specificity and the data are accurate.

Applications

• Singleplex to 4-plex RT-qPCR detection.

• High-copy and low-copy gene detection.

• RNA templates with high GC content or complex secondary structure.

• Detection of RNA virus or trace amounts of RNA.

Storage

Store at -20°C for two years

Shipping

Dry ice (-70 ℃)