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Tris-Borate-EDTA buffer 10X
Catalog Number: B2015100 (1 L)
Tris-Borate-EDTA Buffer 10X is a high quality TBE (Tris/Borate/EDTA) buffer commonly used in nucleic acid electrophoresis. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.
Live enquiry about this product via Text/SMS: 1-858-900-3210.
Product Description
Tris-Borate-EDTA Buffer 10X
Catalog number: B2015100
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 L
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: RT
Keywords: TBE buffer
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.
References:
1: Poduslo JF. Glycoprotein molecular-weight estimation using sodium dodecyl sulfate-pore gradient electrophoresis: comparison of tris-glycine and tris-borate-EDTA buffer systems Anal Biochem. 1981 Jun;114(1):131-9.
2: Stellwagen NC. Apparent pore size of polyacrylamide gels: comparison of gels cast and run in Tris-acetate-EDTA and Tris-borate-EDTA buffers Electrophoresis. 1998 Jul;19(10):1542-7.
3: CHERNOFF AI, PETIT N, NORTHROP J. THE AMINO ACID COMPOSITION OF HEMOGLOBIN. V. THE PREPARATION OF PURIFIED HEMOGLOBIN FRACTIONS BY CHROMATOGRAPHY ON CELLULOSE EXCHANGERS AND THEIR IDENTIFICATION BY STARCH GEL ELECTROPHORESIS USING TRIS-BORATE-EDTA BUFFER Blood. 1965 May;25:646-61.
4: Stellwagen NC, Bossi A, Gelfi C, Righetti PG. DNA and buffers: are there any noninteracting, neutral pH buffers? Anal Biochem. 2000 Dec 1;287(1):167-75.
5: Mayeda A, Krainer AR. Long-term storage of concentrated tris-borate-EDTA electrophoresis buffers without precipitation Biotechniques. 1991 Feb;10(2):182.
6: Stellwagen NC, Gelfi C, Righetti PG. DNA and buffers: the hidden danger of complex formation Biopolymers. 2000 Aug;54(2):137-42.
7: Strutz K, Stellwagen NC. Do DNA gel electrophoretic mobilities extrapolate to the free-solution mobility of DNA at zero gel concentration? Electrophoresis. 1998 May;19(5):635-42.
8: Yakhnin AV, Yakhnin H, Babitzke P. Gel mobility shift assays to detect protein-RNA interactions Methods Mol Biol. 2012;905:201-11.
9: Sanderson BA, Araki N, Lilley JL, Guerrero G, Lewis LK. Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis Anal Biochem. 2014 Jun 1;454:44-52.
10: Schwinefus JJ, Bloomfield VA. The greater negative charge density of DNA in tris-borate buffers does not enhance DNA condensation by multivalent cations Biopolymers. 2000 Dec;54(7):572-7.
Products Related to Tris-Borate-EDTA buffer 10X can be found at Chemicals
Additional Information
Weight | 35 oz |
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Dimensions | 8 × 8 × 8 in |