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Immunohistochemical detection of Tyrosine Hydroxylase in rat brain Substantia Nigra. Brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Tyrosine Hydroxylase at 2 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Neuronal nuclei were counterstained with DAPI (blue)
Immunohistochemical detection of Tyrosine Hydroxylase in rat brain Substantia Nigra. Brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Tyrosine Hydroxylase at 2 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Neuronal nuclei were counterstained with DAPI (blue)