VEGFA Chemi-Luminescent ELISA Kit (Dog)
Cat# OKCD04020
Size : 96Wells
Marca : Aviva Systems Biology
Contatta il distributore locale :
Telefono : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for VEGFA Chemi-Luminescent ELISA Kit (Dog) (OKCD04020) |
|---|
| Predicted Species Reactivity | Canis lupus|Dog | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | CLIA | ||||||||||||||||||||||
| ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
| ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
| ELISA Kit Linearity |
| ||||||||||||||||||||||
| ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vascular Endothelial Growth Factor A (VEGFA) level in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 2.74-2,000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
| ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 2.74 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | VEGFA |
|---|---|
| Gene Full Name | vascular endothelial growth factor A |
| Alias Symbols | vascular endothelial growth factor 188;vascular endothelial growth factor A;vascular permeability factor;VEGF;VEGF-A;VPF. |
| NCBI Gene Id | 403802 |
| Protein Name | Vascular endothelial growth factor A |
| Description of Target | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). |
| Uniprot ID | Q9MYV3 |
| Protein Accession # | NP_001003175 |
| Nucleotide Accession # | NM_001003175 |