Vitamin B12 ELISA Kit

Cat# OKEH02574

Size : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02574
Product Info
Predicted Species ReactivityAll Species
ApplicationELISA-Comp
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Vitamin B12 ELISA Kit (OKEH02574) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Vitamin B12 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vitamin B12 and incubated. The Vitamin B12 found in the sample or standards competes with the biotinylated Vitamin B12 for limited binding sites on the immobilized anti-Vitamin B12 antibody. Excess unbound biotinylated Vitamin B12 and sample or standard Vitamin B12 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vitamin B12 captured in the well and inversely proportional to the amount of Vitamin B12 which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Vitamin B12 Microplate96 Wells (12 x 8 Well strips)
Vitamin B12 Lyophilized Standard2 x 10 ng
Sample Diluent1 x 20 mL
100X Vitamin B12-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: VB12
Target Name: Vitamin B12
::Pubchem: 5479203
::Chemical formula: C63H88CoN14O14P
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.051 ng/mL
SpecificityNatural and recombinant General Vitamin B12
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsVB12, Vitamin B12, VB12, cobalamin
Protein NameVitamin B12
Description of TargetIt is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid synthesis and energy production. Vitamin B12, vitamin B12 or vitamin B-12, also called cobalamin, is a water-soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood. It is one of the eight B vitamins.

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Cat#
Descrizione
Cond.
Priced
OKEH02570
 96Wells