- More Files
Hybridization position of the probes on the chromosome.
Hybridization position of the probes on the chromosome.
- Specifications
Product Description
Labeled FISH probes for identification of gene split using Fluorescent In Situ Hybridization Technique. ().
Probe 1
Name: WT1
Size: Approximately 390kb
Fluorophore: Texas Red
Location: 11p13Probe 2
Name: WT1
Size: Approximately 600kb
Fluorophore: FITC
Location: 11p13Origin
Human
Source
Genomic DNA
Reactivity
Human
Notice
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulatory Status
For research use only (RUO)
Quality Control Testing
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction
Store at 4°C in the dark.
Note
Hybridization position of the probes on the chromosome.
- Applications
Fluorescent In Situ Hybridization (Cell)
- Gene Info — WT1
Entrez GeneID
7490Gene Name
WT1
Gene Alias
GUD, WAGR, WIT-2, WT33
Gene Description
Wilms tumor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
Other Designations
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- Interactomes
- Diseases