3,3′,5,5′-Tetramethylbenzidine (TMB) is another widely used colorimetric substrate in Western blotting, particularly for detecting horseradish peroxidase (HRP)-conjugated antibodies. Its ability to produce a blue precipitate upon enzymatic reaction makes it a valuable tool in protein analysis.

Key Features of TMB

• Rapid Signal Development: Produces results within minutes.

• High Contrast: The blue precipitate offers excellent visibility against the membrane background.

• Compatibility: Works seamlessly with nitrocellulose and PVDF membranes.

Applications in Research

• Protein Quantification:

  • Used for detecting proteins in diverse biological samples.

• Educational Demonstrations:

  • Simplifies protein detection protocols for teaching purposes.

Advantages of TMB

• Simple and straightforward protocol.

• Cost-efficient, making it suitable for routine applications.

• High contrast signals ideal for qualitative analysis.