Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
RT-qPCR mix for probes High ROX

RT-qPCR mix for probes High ROX

High ROX quantitative RT-PCR mixes for probes are made for use with qPCR devices requiring high ROX as an internal reference dye for fluorescent signal normalization and correction of optical well-to-well variations.
The mix for probes consists of all the necessary components (except template DNA, primers and probes): a Reverse transcriptase, a Taq polymerase, dNTPs, MgCl2 and High ROX dye.


Here's how to select the appropriate ROX dye for the qPCR device you are using:

UniversalAll instruments
Without ROXBio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4;
Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s;
Illumina Eco qPCR;
Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000;
Roche Applied Science LightCyclerTM 480;
Thermo Scientific PikoReal Cycler.
Low ROXApplied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000,
MX3005P, MX3000P
High ROXApplied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast;
StepOne, StepOnePlus

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