Sabouraud dextrose broth - Selective liquid media for microbiology
Sabouraud dextrose broth (Sabouraud liquid medium) is used for the culture of yeasts, moulds and aciduric micro-organisms. Sabouraud Dextrose Broth is recommended for the detection of Candida albicans in non-sterile products. Sabouraud dextrose media are peptone media supplemented with dextrose to promote the growth of fungi. Special peptone provides a source of carbon and nitrogen, vitamins, minerals, amino acids and growth factors. Dextrose provides a source of energy for the growth of microorganisms. The low pH promotes the growth of fungi and inhibits contaminating bacteria in clinical specimens. The acidic reaction of the final medium is inhibitory to a large number of bacteria, making it particularly useful for the cultivation of acidic fungi and microorganisms. To isolate fungi from contaminated specimens, a selective medium must be inoculated simultaneously. Incubate the cultures for 4-6 weeks before reporting them as negative.
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