SDS precast gels with a 4-12% gradient are essential tools in protein electrophoresis, allowing for effective separation of proteins based on their molecular weight. These gels feature a gradient that ranges from 4% at the top to 12% at the bottom, enabling the resolution of a wide array of protein sizes in a single run.

Key Advantages

1. Optimized Separation: The gradient structure allows larger proteins to migrate more freely at the top, while smaller proteins face greater resistance as they move toward the bottom. This design enhances the resolution of mixed protein samples.
2. Convenience and Consistency: Being precast, these gels eliminate the variability associated with hand-casting, ensuring consistent results across experiments. This convenience saves time and reduces preparation errors in laboratory workflows.
3. Compatibility: Many 4-12% gradient gels are compatible with standard electrophoresis systems. This compatibility facilitates easy integration into existing lab protocols.
4. Performance: Gels offer improved separation efficiency and faster run times compared to traditional Laemmli gels, providing clear and distinct bands necessary for accurate analysis.

Applications

These gels are widely used in various applications, including:

• SDS-PAGE: For analyzing protein samples under denaturing conditions.
• Western Blotting: Following electrophoresis, proteins can be transferred to membranes for further analysis.
• Protein Characterization: Ideal for assessing protein purity and molecular weight.