Anti-PGR antibody (320-370) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.50 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:150
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	PGR
Gene ID:	5241
Uniprot ID:	PRGR_HUMAN
Immunogen Region:	320-370
Immunogen:	Synthetic peptide taken within amino acid region 320-370 on human Progesterone receptor protein.

Tissue Specificity	In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. Isoform A and isoform B are expressed in comparable levels in uterine glandular epithelium during the proliferative phase of the menstrual cycle. Expression of isoform B but not of isoform A persists in the glands during mid-secretory phase. In the stroma, isoform A is the predominant form throughout the cycle. Heterogeneous isoform expression between the glands of the endometrium basalis and functionalis is implying region-specific responses to hormonal stimuli.
Post Translational Modifications	Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1. Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294. Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294. Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
Function	The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor. Isoform A: Ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity including repression of its isoform B, MR and ER. Transrepressional activity may involve recruitment of corepressor NCOR2. Isoform B: Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation. Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
Protein Name	Progesterone Receptor Pr Nuclear Receptor Subfamily 3 Group C Member 3
Database Links	Reactome: R-HSA-1251985 Reactome: R-HSA-3371497 Reactome: R-HSA-383280 Reactome: R-HSA-4090294 Reactome: R-HSA-9018519
Cellular Localisation	Nucleus Cytoplasm Nucleoplasmic Shuttling Is Both Hormone- And Cell Cycle-Dependent On Hormone Stimulation Retained In The Cytoplasm In The G(1) And G(2)/M Phases Isoform A: Nucleus Mainly Nuclear Isoform 4: Mitochondrion Outer Membrane
Alternative Antibody Names	Anti-Progesterone Receptor antibody Anti-Pr antibody Anti-Nuclear Receptor Subfamily 3 Group C Member 3 antibody Anti-PGR antibody Anti-NR3C3 antibody

Information sourced from Uniprot.org

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