Anti-Phospho-FGFR2 antibody (520-570) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.55-0.75 µg/µl
Dilution Range:	WB: 1:500 ELISA: 1:10, 000 IP: 1:150
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	FGFR2
Gene ID:	2263
Uniprot ID:	FGFR2_HUMAN
Immunogen Region:	520-570
Immunogen:	Synthetic peptide taken within amino acid region 520-570 on human Fibroblast Growth Factor receptor 2 protein.

Post Translational Modifications	Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on several tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-769 is essential for interaction with PLCG1. N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus. Ubiquitinated. FGFR2 is rapidly ubiquitinated after autophosphorylation, leading to internalization and degradation. Subject to degradation both in lysosomes and by the proteasome.
Function	Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.
Protein Name	Fibroblast Growth Factor Receptor 2 Fgfr-2 K-Sam Kgfr Keratinocyte Growth Factor Receptor Cd Antigen Cd332
Database Links	Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-190375 Reactome: R-HSA-190377 Reactome: R-HSA-2023837 Reactome: R-HSA-2033519 Reactome: R-HSA-2219530 Reactome: R-HSA-5654221 Reactome: R-HSA-5654695 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654727 Reactome: R-HSA-5655253 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-8851708 Reactome: R-HSA-8853333
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Golgi Apparatus Cytoplasmic Vesicle Detected On Osteoblast Plasma Membrane Lipid Rafts After Ligand Binding The Activated Receptor Is Rapidly Internalized And Degraded Isoform 1: Cell Membrane Isoform 3: Cell Membrane Isoform 8: Secreted Isoform 13: Secreted
Alternative Antibody Names	Anti-Fibroblast Growth Factor Receptor 2 antibody Anti-Fgfr-2 antibody Anti-K-Sam antibody Anti-Kgfr antibody Anti-Keratinocyte Growth Factor Receptor antibody Anti-Cd Antigen Cd332 antibody Anti-FGFR2 antibody Anti-BEK antibody Anti-KGFR antibody Anti-KSAM antibody

Information sourced from Uniprot.org

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