ELISA: The antibody may be used as a detection antibody in ELISA techniques in combination with SM1498P as capture antibody. Western Blotting: Under reducing conditions, this antibody detects a band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Reactivity
Canine, Human
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
Specificity
This Mouse anti Dog IgE antibody, clone E6-71A1 recognizes Canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of 62-65kDa under reducing conditions.
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