CD3 antibody [OKT3] (PerCP-Cy5.5)
Cat# GTX79906-11-100test
Size : 100test
Marca : Genetex
Contatta il distributore locale :
Telefono : +1 850 650 7790
![Anti-CD3 antibody [OKT3] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX79906-11](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-CD3 antibody [OKT3] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX79906-11")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | OKT3 |
Isotype | IgG2a |
Application | FACS |
Reactivity | Human, Chimpanzee |
| Package | 100 test |
Datasheet File View product citations for antibody GTX79906-11 on CiteAb
PRODUCT
Summary
The OKT3 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test |
Not tested in other applications.
Product Note
This antibody recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.05 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Commercially sensitive information
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3d molecule , CD3e molecule , CD3g molecule , CD3-DELTA , IMD19 , T3D , IMD18 , T3E , TCRE , CD3-GAMMA , IMD17 , T3G
Cellular Localization
Cell membrane; Single-pass type I membrane protein , Cell membrane
Database
DATA IMAGES
| | GTX79906-11 FACS Image FACS analysis of human peripheral blood lymphocytes using GTX79906-11 CD3 epsilon antibody [OKT3] (PerCP-Cy5.5). |
Clone Reference
Zheng G et al. Signal Transduct Target Ther 2021; 6 (1):236 Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab.
Zhang C. et al. J Surg Oncol 2021; (Epub) Histological tumor response to neoadjuvant chemotherapy correlates to Immunoscore in colorectal cancer liver metastases patients.
2019; Contribution of ROS and metabolic status to neonatal and adult CD8+ T cell activation
2018; STAT5B: A Differential Regulator of the Life and Death of CD4 + Effector Memory T Cells
2012; Incorporation of Transmembrane Hydrophobic Mutations in the TCR Enhance Its Surface Expression and T Cell Functional Avidity
Submit a Reference REVIEW
Clone Reference
Zheng G et al. Signal Transduct Target Ther 2021; 6 (1):236 Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab.
Zhang C. et al. J Surg Oncol 2021; (Epub) Histological tumor response to neoadjuvant chemotherapy correlates to Immunoscore in colorectal cancer liver metastases patients.
2019; Contribution of ROS and metabolic status to neonatal and adult CD8+ T cell activation
2018; STAT5B: A Differential Regulator of the Life and Death of CD4 + Effector Memory T Cells
2012; IL-7 drives Th1 and Th17 cytokine production in patients with primary SS despite an increase in CD4 T cells lacking the IL-7Rα
2012; Incorporation of Transmembrane Hydrophobic Mutations in the TCR Enhance Its Surface Expression and T Cell Functional Avidity
2011; A novel adoptive transfer model of chronic lymphocytic leukemia suggests a key role for T lymphocytes in the disease
2009; A new approach for quantitative phosphoproteomic dissection of signaling pathways applied to T cell receptor activation
2008; The quality of chimpanzee T-cell activation and simian immunodeficiency virus/human immunodeficiency virus susceptibility achieved via antibody-mediated T-cell receptor/CD3 stimulation is a function of the anti-CD3 antibody isotype
2005; CD1a and CD1c activate intrathyroidal T cells during Graves' disease and Hashimoto's thyroiditis
1986; The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining
| SDS | |
|---|---|
| PBS.pdf | |
| Sodium Azide.pdf | |