CXCL10 ELISA Kit (Human)

Cat# OKEH00401

Size : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00401
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CXCL10 ELISA Kit (Human) (OKEH00401) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CXCL10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human CXCL10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CXCL10 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 9.3% (n = 20)
ELISA Kit Component
ComponentAmount
CXCL10 Microplate96 Wells (12 x 8 Well strips)
CXCL10 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CXCL101 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human C-X-C motif chemokine 10
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCXCL10
Gene Full NameC-X-C motif chemokine ligand 10
Alias Symbols10 kDa interferon gamma-induced protein;C7;crg-2;C-X-C motif chemokine 10;gamma IP10;gIP-10;IFI10;INP10;interferon-inducible cytokine IP-10;IP-10;mob-1;protein 10 from interferon (gamma)-induced cell line;SCYB10;small inducible cytokine subfamily B (Cys-X-Cys), member 10;small-inducible cytokine B10.
NCBI Gene Id3627
Protein NameP02778
Description of TargetC-X-C motif chemokine 10
Uniprot IDhttps://www.uniprot.org/uniprot/P02778
Protein Accession #NP_001556.2
Nucleotide Accession #NM_001565.3
Protein Size (# AA)98
Molecular Weight10 kDa

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