EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Applications: |Suitable for use in Western Blot and Immunohistochemistry.|Other applications not tested.||Recommended Dilutions:|Western Blot: 0.05ug/ml detected EOMES in 10ug of E13-14 mouse brain lysates.|Immunohistochemistry (Paraffin): 1:100 from a previous lot detected EOMES in mouse and human brain. Requires HIER with citrate buffer, pH 6.0|Immunohistochemistry (Paraffin): 1:1000 from a representative lot detected EOMES in mouse cerebral cortex and mouse cerebellum tissue. Requires HIER with citrate buffer, pH 6.0|Optimal dilutions to be determined by the researcher.||Positive Control:|E13-14 mouse brain lysate||Recommended Secondary Antibodies:|I1906-10: IgY, Chicken (HRP) Rb x Ch |I1906-12: IgY, Chicken (FITC) Rb x Ch |I1906-14: IgY, Chicken (AP) Rb x Ch||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.