Ethidium bromide 99+%

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10ug/ml). Ethidium bromide has been used in a number of fluorometric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).||Synonyms:|3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide; 2,7-Diamino-10-ethyl-9-|phenylphenanthridinium Bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium Bromide; 2,7-Diamino-9-phenylphenanthridine Ethobromide; Dromilac; Homidium Bromide||CAS No:|1239-45-8||Molecular Formula:|C21H20BrN3 ||Molecular Weight:|394.31||Appearance:|Dark red to purple color powder||Purity:|≥99% (HPLC)||IR:|Conforms to reference||Melting Point:|260-262°C||Iron:|To pass test||pH:|4-7||Loss on Drying:|≤10%||Sulfated Ash:|≤0.2%||Related Substances:|To pass test||Solubility:|Water||Storage and Stability:|May be stored at RT.