Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Glucose Uptake Fluorometric Assay Kit

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Size : 500Assays

Telefono : +1 850 650 7790

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Telefono : +1 850 650 7790

Detection Principle	2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP⁺ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.
Sample Type	Cell
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=530 nm/590 nm)
Research Area	Metabolic Diseases , Glycolysis And Lipid Metabolism , Disulfidptosis
Other Reagents Required	KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4)
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Assay Time	1 h 40 min

Cat.No.	Product Name	Sizes
E-BC-K234-M	Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)	500Assays , 96T , 48T

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Fluorescence microplate reader

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