P1015-500ug | IFN-gamma, murine recombinant Clinisciences

Background

Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. It shares high sequence indentity with rat IFN-γ (86 %). IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation.

Reference:

1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol

2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55

3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56

4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.

Description

Gene ID	15978
Accession #	P01580
Alternate Names
Source	Escherichia coli.
M.Wt	Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.
AA Sequence	HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRC
Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Formulation	Lyophilized from a 0.2 µm filtered solution in 20 mM Tris-HCl pH8.0, 300 mM NaCl, containing 5 % trehalose, 0.05 % Tween-20.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled 20 mM Tris-HCl pH8.0 with 50 mM NaCl to a concentration of 0.1-0.3 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of > 1.3 × 10⁶ IU/mg.
Shipping Condition	Gel pack.
Handling	Centrifuge the vial prior to opening.
Usage	For Research Use Only! Not to be used in humans.

Product Citation

1. Yuanping Li, Dan Zheng, et al. "Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M2 Polarization." J Immunol Res. 2020 Oct 10;2020:2462039. PMID: 33102606
2. Xiao-yan Zhou, Rong Gao, et al. "Trained Innate Immunity by Repeated Low-Dose Lipopolysaccharide Injections Displays Long-Term Neuroprotective Effects." Mediators Inflamm. 2020 Oct 1;2020:8191079. PMID: 33061831

Quality Control

Purity > 96 % by SDS-PAGE and HPLC analyses.
Endotoxin: Less than 1 EU/µg of rMuIFN-γ as determined by LAL method.

Datasheet

Related Biological Data

IFN-gamma, murine recombinant

Cat# P1015-500ug

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