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> IgG2a, Heavy Chain (APC)

IgG2a, Heavy Chain (APC)

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Cat# I1904-83A-APC-100ul

Size : 100ul

Marca : US Biological

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Telefono : +1 850 650 7790

I1904-83A-APC Rabbit Anti-IgG2a, Heavy Chain (APC)

Clone Type
Polyclonal
Host
rat
Source
mouse
Swiss Prot
P01863, P01865, P01864
Isotype
IgG1
Grade
Affinity Purified
Applications
FLISA
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Applications:|Suitable for use in FLISA. Other applications not tested.||Recommended Dilutions:|FLISA: coating antibody.|Optimal working dilutions to be determined by researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2371 (LO-MG2a-7)|Host: rat|Source: mouse|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by affinity chromatography from tissue culture supernatant.|Immunogen: Purified IgG from BALB/c mice.|Specificity: Recognizes the mouse gamma 2a immunoglobulin heavy chain. Recognizes an allotypic determinant upon mouse IgG2a. Reacts with the IgHIa allotype (as expressed in BALB/c mice). Does not react with the IgHIb allotype (as expressed in C57/BL mice).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified IgG from BALB/c mice.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the mouse gamma 2a immunoglobulin heavy chain. Recognizes an allotypic determinant upon mouse IgG2a. Reacts with the IgHIa allotype (as expressed in BALB/c mice). Does not react with the IgHIb allotype (as expressed in C57/BL mice).
References
1. Jarman, E., et al., Immunology 112: 631-642 (2004). 2. Bazin, H., (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids. 615-618. Pergamon Press, Oxford and New York. 3. Bazin, H., et al., J. Immunol. Methods 66: 261-269. (1984) 4. Bazin, H., et al., J. Immunol. Methods. 71: 9-16 (1984). 5. Querinjean, P., et al., Eur. J. Biochem. 31: 354-359 (1972). 6. Nagao, K., et al., Am. J. Respir. Cell. Mol. Biol. 29: 314-320 (2003). 7. Ramos, J.D.A., et al., Philippine Science Letters 2: 38-48 (2009). 8. Ormstad, H., et al., Toxicology 188: 309-318 (2003). 9. Ormstad, H., et al., J. Toxicol. Environ. Health A 61: 55-67 (2000). 10. Hall, G., et al., Vaccine 21: 549-561 (2003). 11. Tan, L.K., et al., Vaccine 24: 5762-5771 (2006). 12. Instanes, C. & Hetland, G., Toxicology 204: 13-21 (2004). 13. Lieden, A., et al., Allergy 64: 304-311 (2009). 14. Wolfowicz, C.B., et al., Vaccine 21: 1195-1204 (2003).