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Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Lama-84

Stampa

Cat# 300261

Size : 1cryovial

Contatta il distributore locale :

Telefono : +1 850 650 7790

Marca : CLS Cell Lines Service

Contatta il distributore locale :

Telefono : +1 850 650 7790

Lama-84 Cells

Description	LAMA-84 is a human cell line derived from the peripheral blood of a patient with chronic myeloid leukemia (CML) in blast crisis. This cell line is characterized by the presence of the Philadelphia chromosome, which results in the BCR-ABL fusion gene, a hallmark of CML. The BCR-ABL oncogene is known for its role in increasing tyrosine kinase activity, which promotes various signaling pathways leading to uncontrolled cell proliferation and resistance to apoptosis. LAMA-84 cells are thus an invaluable model for studying the molecular mechanisms of CML progression and for evaluating the efficacy of tyrosine kinase inhibitors (TKIs) in a pre-clinical setting. In research, LAMA-84 has been extensively used to understand the biology of CML, especially in the context of drug resistance and disease evolution. Studies involving this cell line have helped in elucidating the cellular responses to different generations of TKIs, such as imatinib, dasatinib, and nilotinib. Moreover, LAMA-84 has contributed to the investigation of new therapeutic strategies aimed at overcoming TKI resistance, including the testing of combination therapies that target other signaling pathways synergistically affected by the BCR-ABL fusion protein.
Organism	Human
Tissue	Blood
Disease	Chronic myeloid leukemia
Synonyms	LAMA-84, LAMA84, Lama84

Age	29 years
Gender	Female
Ethnicity	Caucasian
Morphology	Round cells
Growth properties	Suspension, some adherent cells

Citation	Lama-84 (Cytion catalog number 300261)
Biosafety level	1

Surface antigens	GPIIb/IIIa+, GPIIIa+
Viruses	EBNA, EA, and VCA were not detected
Mutational profile	BCR-ABL1 pos

Culture Medium	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
Medium supplements	Supplement the medium with 10% FBS
Doubling time	30 hours
Subculturing	Cells adhering to the bottom of the cell culture flask may be dislodged by shaking. Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth.
Split ratio	A ratio of 1:2 to 1:3 is recommended
Seeding density	1 to 2 x 10^4 cells/cm^2
Freezing recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Optionally, skip centrifugation but remove any remaining freezing medium after 24 hours. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
STR profile	CSF1PO: 11,12,13 D13S317: 11 D16S539: 11 D5S818: 11,12 D7S820: 11 TH01: 6,7 TPOX: 10 vWA: 14,17 D3S1358: 14,17 D21S11: 29,30,31 D18S51: 13 Penta E: 7 Penta D: 10 D8S1179: 10,15 FGA: 21,22 D1S1656: 15,15.3 D6S1043: 10,20 D2S1338: 17 D12S391: 18,24 D19S433: 13
HLA alleles	A: 02:01:01, 25:01:01 B: 18:01:01, 44:02:01 C: 05:01:01, 12:03:01 DRB1: 04:02:01, 15:01:01G DQA1: 01:02:01, 03:01:01 DQB1: 03:02:01, 06:02:01 DPB1*: 09:01:01, 23:01:01 E: 01:01:01