LDH5 M4 Isozyme Rabbit Polyclonal Antibody
Cat# AP21331AF-N
Size : 10mg
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LDH5 M4 Isozyme Rabbit Polyclonal Antibody
SKU
AP21331AF-N
LDH5 M4 Isozyme rabbit polyclonal antibody, Azide Free
| Product Data | |
| Application | ELISA, ID, IF, IP, R, WB |
|---|---|
| Application | This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/5,000). |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | L-Lactic dehydrogenase, LDH-5(M4) isoenzyme is isolated and purified from Human placenta. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
| Buffer | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized IgG fraction. |
| Reconstitution Method | Restore by adding 1.0 ml sterile distilled water. |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography. |
| Conjugation | Unconjugated |
| Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
| Stability | Shelf life: one year from despatch. |
| Background | Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes. |
| Synonyms | L-Lactic dehydrogenase LDH-5 Isozyme M4 |
| Reference Data | |
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